The Mechanobiology & Soft Matter group seeks to understand the basic physical principles underlying force transmission and elucidating how cell mechanical properties regulate cellular functions. Our experimental approach of cell mechanics takes advantage of microfabricationd and force assays to address physiological questions at single-cell and tissue levels. Among different reserach projects, the Mechanobiology & Soft Matter group is interested on epithelial cell migration, inside-out and outside-in mechanotransduction pathways and force propagation in neuronal networks.
We have an expertise in microfabrication (2D micropatterns and 3D microchannels), traction force microscopy (TFM), confocal imaging of the cell cytoskeleton, cell stretching, cell confinement assays (2D and 3D), soft hydrogels (flat and microstructured) and mechanical properties of cells and culture substrates (microindentation in liquid).
We look forward collaborating with groups that works on new theoretical and biological models. Theoretical models could be highly valuable to support our experimental data on collective cell migration. We are also interested to collaborate with groups that work with super-resolution microscopy (STORM, STED or SIM). We are interested to collaborate with groups that have developed stable GFP-cell lines (e.g. actin, cadherin and vinculin) for cell migration assays, such as MDCK cell lines, epithelial keratocytes (zebrafish) or neuroblastoma (SH-SY5Y). We have also a deep interest in nuclear mechanics and functions, as well as gene expression in relation with mechanotransduction pathways.
1- Substrate area confinement is a key determinant of cell velocity in collective migrationdepositphotos_108313632-stock-illustration-download-pdf-file-button
D. Mohammed, G. Charras, E. Vercruysse, M. Versaevel, J. Lantoine, L. Alaimo, C. Bruyère, M. Luciano, K. Glinel, G. Delhaye, O. Théodoly and S. Gabriele
Nature Physics 15, 858-866 (2019)
2- Actomyosin contractility scales with myoblast elongation and enhances differentiation through YAP nuclear export depositphotos_108313632-stock-illustration-download-pdf-file-button
C. Bruyère, M. Versaevel, D. Mohammed, L. Alaimo, M. Luciano, E. Vercruysse and S. Gabriele
Scientific Reports 9, 15565 (2019)
3- Matrix stiffness modulates formation and activity of neuronal networks of controlled architecturesdepositphotos_108313632-stock-illustration-download-pdf-file-button
J. Lantoine, T. Grevesse, A. Villers, G. Delhaye, C. Mestdagh, M. Versaevel, D. Mohammed, C. Bruyère, L. Alaimo, S.P. Latour, L. Ris and S. Gabriele
Biomaterials 89, 14-24 (2016)
4- Opposite rheological properties of neuronal microcompartments predict axonal vulnerability in brain injuriesdepositphotos_108313632-stock-illustration-download-pdf-file-button
T. Grevesse, B.E. Dabiri, K.K. Parker and S. Gabriele
Scientific Reports, 5, 9475 (2015)
5- Spatial cordination between cell and nuclear shape within micropatterned endothelial cellsdepositphotos_108313632-stock-illustration-download-pdf-file-button
M.Versaevel, T. Grevesse and S. Gabriele
Nature Communications, 3, 671 (2012)
9-11 September 2019, Trnava, Slovakia