I am interested in the role of phase separation for gene expression regulation / gene activation. This includes understanding the mechanism behind this process which we are trying to uncover using biochemical and biophysical in vitro assays, combined with in vivo imaging and gene expression analysis.
various RNA techniques, phase separation assays, fluorescent imaging, recombinant expression and purification of proteins, construct and mutant design
advanced (super-resolution) fluorescent imaging
Wittmann S, Renner M, Watts BR, Adams O, Huseyin M, Baejen C, El Omari K, Kilchert C, Heo DH, Kecman T, Cramer P, Grimes JM, Vasiljeva L. 2017. The conserved termination factor Seb1 bridges RNA polymerase II and nascent RNA. Nat Commun 8: 14861.
Wittmann S, Alberti S. 2019. ERα condensates: chronic stimulation is hard to ignore. Nat Struct Mol Biol 26(3):153-154.
25–30 May 2020, Spetses Island, Greece