Our research focuses on the gene expression pathway, and specifically on mRNA dynamics in living cell systems. We study dynamic cell processes on the single-molecule, single-gene and single-cell level using time-lapse fluorescent microscopy and subsequent kinetic analysis.
The primary goals of our research are to understand how genes switch "on" and "off" in normal cells and in cancer cells, how quickly are mRNAs transcribed, the kinetics of the transcription process in vivo, and their travels and destinations as they translocate within the cell under normal conditions and during stress.
High resolution imaging in fixed.
Time lapse imaging in living cells
Aizer et al (2014). Quantifying mRNA targeting to P bodies in living human cells reveals their dual role in mRNA decay and storage. J. Cell Sci. 127, 4443.
Kalo et al (2015). Cellular levels of signaling factors are sensed by β-actin alleles to modulate transcriptional pulse intensity. Cell Rep. 11, 419.
Kafri et al (2016). Quantifying β-catenin subcellular dynamics and cyclin D1 mRNA transcription during Wnt signaling in single living cells. eLIFE e16748.
Sheinberger et al (2017). CD-tagging-MS2: detecting allelic expression of endogenous mRNAs and their protein products in single cells. Biol. Methods Protoc., 2; 1.
Avivi et al (2017). Visualizing nuclear RNAi activity in single living human cells. Proc. Natl. Acad. Sci. USA 114, E8837.
Yunger et al (2018). S-phase transcriptional buffering quantified on two different promoters. Life Sci. Alli.1, e201800086.
Hochberg-Laufer et al (2019). Uncoupling of nucleo-cytoplasmic RNA export and localization during stress. Nucleic Acids Res. 47, 4778.
Greenberg et al (2019). Cytoplasmic DNA can be detected by RNA fluorescence in situ hybridization. Nucleic Acids Res. In press.
Ben-Yishay et al (2019). Imaging within single NPCs reveals NXF1’s role in mRNA export on the cytoplasmic side of the pore. J. Cell Biol. 218, 2962.