Our research addresses the role of lamin A in the regulation of pathophysiological processes and the mechanisms that accelerate cardiovascular disease and aging in patients with Hutchinson-Gilford progeria syndrome (HGPS), a rare genetic disease caused by progerin, a mutant form of lamin A which is also expressed at low level in tissues from non-HGPS subjects. Our experimental strategy involves a multifaceted approach that combines in vitro, cellular, animal and human studies and a variety of technologies, including mouse genetic engineering, proteomics, transcriptomics, FRET, confocal microscopy, in vivo invasive and non-invasive imaging.
Molecular and cellular techniques and methodologies to study cardiovascylar disease using cellular and animal models and analysis of human samples.
Single cells genomics.
Hamczyk MR, Villa-Bellosta R, Quesada V, Gonzalo P, Vidak S, Nevado RM, Andrés-Manzano MJ, Misteli T, López-Otín C*, Andrés V*. Progerin accelerates atherosclerosis by inducing endoplasmic reticulum stress in vascular smooth muscle cells. EMBO Mol Med 11(4).pii:e9736. doi: 10.15252/emmm.201809736 (2019).
Hamczyk MR, Villa-Bellosta R, Gonzalo P, Andrés-Manzano MJ, Nogales P, Bentzon JF, López-Otín C, Andrés V. Vascular smooth muscle-specific progerin expression accelerates atherosclerosis and death in a mouse model of Hutchinson-Gilford progeria syndrome. Circulation 138:283-286 (2018)
Fernández-Alvira JM, Fuster V*, Dorado B, Soberón N, Flores I, Gallardo M, Pocock S, Blasco MA & Andrés V*. Short telomere load, telomere length and subclinical atherosclerosis in the PESA study. J Am Coll Cardiol 67:2467-2476 (2016)
Chèvre R, González-Granado JM, Megens RTA, Sreeramkumar V, Silvestre-Roig C, Molina-Sánchez P, Weber C, Soehnlein O, Hidalgo A*, Andrés V*. High-resolution imaging of intravascular atherogenic inflammation in live mice. Circ Res 114:770-779 (2014) (Issue cover)
Villa-Bellosta R, Rivera-Torres J, Osorio FG, Acín-Pérez R, Enriquez JA, López-Otín C, Andrés V. Defective extracellular pyrophosphate metabolism promotes vascular calcification in a mouse model of Hutchinson-Gilford progeria syndrome that is ameliorated on pyrophosphate treatment. Circulation 127:2442-2451 (2013)
25–30 May 2020, Spetses Island, Greece