We investigate the role of Intermediate filaments, especially vimentin, on biophysical properties of cells, such as cell migration, stiffness and polarity. We are further interested in the interplay of vimentin with actin and microtubules. Finally, we are investiagting the structure and dynamics of the actin cortex and microtentacles in suspended cells.
High resolution microscopy techniques (Confocal, SEM),
Laser manipulation techniques: Fluorescent Recovery After Photobleaching (FRAP), Laser Ablation
Migration assays of both strongly and weakly adherent cells (e.g. microchambers for immune cell migration)
Cell mechanical properties for both adherent and non adherent cells
specific drugs targeting IFs
Danielsson F., K.P. McKenzie, H. Caldeira Araujo, F. Lautenschläger, A.K.B. Gad (2018) “Vimentin Diversity in Health and Disease” Cells, 7(10)
Terriac, E., G. Coceano, Z. Mavajian, T. A. Hageman, A. F. Christ, I. Testa, A. K. Gad, and F. Lautenschlager (2017). "Vimentin Levels and Serine 71 Phosphorylation in the Control of Cell-Matrix Adhesions, Migration Speed, and Shape of Transformed Human Fibroblasts." Cells 6(1).
M. Raab, M. Gentilli, H. de Belly, H.R. Thiam, P. Vargas, A.J. Jimenez, F.Lautenschlaeger, A.M. Lennon Duménil, N. Manel, M. Piel (2016) Lamin A/C and ESCRT III promote the survival of migrating cells by ensuring nuclear envelope integrity and preventing DNA damage. Science 352(6283): 359-362.
C. J. Chan, A. E. Ekpenyong, S. Golfier, W. Li, K. J. Chalut, O. Otto., J. Elgeti, J. Guck, F. Lautenschläger (2015) Myosin II activity softens cells in suspension. Biophysical Journal 108(8): 1856-1869.
Y.-J. Liu, M. Le Berre, F. Lautenschläger, P. Maiuri, A. Callan Jones, T. Takaki, R. Voituriez, M. Piel (2015) Confinement in a non-adhesive environment induces fast amoeboid migration of slow mesenchymal cells. Cell 160(4): p. 659-672.
25–30 May 2020, Spetses Island, Greece