Our group is working on neurological diseases with inflammatory etiology. In particular, we are focusing in scaffolding proteins (insulin recpetor substrate, IRS1 and IRS2). mediating trophic factors effect in vivo and in vitro. We also use CRISPR knockout cells generated by collaborators.
In addition, our interest broaden to psichiatric diseases, reward and depression. We are interested in further understanding neuroinflammation and structural proteins in pathological situations. Recently, we have started a study measuring perinuclear nets in our model of neuroinflammation with preliminary, yet interesting results.
Our group uses molecular and cellular biology methods, along with animal models, stereotaxic surgery, behavioral paradigm and postmortem analysis, including ex vivo tests.
We carry out, routinely, protein (western blots) analyiss; quantitaive PCR; and immunohistochemistry immunofluorescence, and confocal microscopy.
We design and clone shRNA; genes and promoters. We can generate AAV derived particules and succesfully inject them by estereotaxia in vivo to study behavioural alterations as a consequence of modulating gene expression.
We are acquantance in behaviour, neuroanatomy, primary cultures, cell lines, and molecular biology.
Primary cultures of neurons and astrocytes and shRNA silencing.
Epigenetic and gene regualtion, bioinformatics
Pathophysiology of extracellular matrix regulation.