Our group is focused on evolutionary processes undergoing Y chromosome degeneration. During my PhD, I am using clasical cytogenetic techniques (immunostaining, FISH...) and new methods in molecular biology (transcriptome modulation of male genome) to study sex chromosomes in model plant Silene latifolia.
cytogenetic techniques - FISH (BAC, "single copy" FISH), plant chromosome preparation for different kinds of subsequent analysis (meiocytes, classical metaphases)
microscopic techniques - FLIM
image processing - moderate skills of ImageJ and Photoshop
3D-SIM, STED, advanced image processing
Bačovský V, Houben A, Kumke K, Hobza R (2018) The distribution of epigenetic histone marks differs between the X and Y chromosomes in Silene latifolia. Planta, in preparation.
Bačovský V, Hobza R, Vyskot B (2018) Technical review: Cytogenetic tools for studying mitotic chromosomes BT – Plant chromatin Dynamics:Methods and Protocols, in: Bremer M, Baroux C, (Eds.). Springer New York, New York, NY, pp. 509 – 535.