Country: Czech Republic
My resesarch focuses on developing microscopic optical contrasting (label-free) modalities to efficiently visualize unstained living cells. These include (1) modulation-relief ("schlieren") microscopy as a competitor of much more costly differential interference contrast (DIC Nomarski) microscopy, and (2) phase-contrast microscopy devoid of 'halo' artifacts otherwise present in commercially available microscopes. Recently I started to engage in (3) Raman microscopy where cell staining is largely contraindicated, and hope to couple it with one of the above-mentioned optical-contrasting modalities [1,2] in order to add a much-needed morphological context to the chemical images Raman microscopy provides.
(1) Modulation-relief ("schlieren") microscopy
(2) Label-free phase-contrast microscopy (under development)
(3) Raman microscopy
Pelc R., Hostounský Z., Otaki T. & Katoh K. (2018 in press)
Conventional, apodized and relief phase-contrast microscopy.
In: Neurohistology & Imaging: Basic Techniques (Pelc R., Doucette J.R. & Walz W., Eds.), pp. XXX-XXX.
(Neuromethods XX: XXX-XXX)
Springer (Humana Press), New York
Pelc R., Hostounský Z. & Otaki T. (2008)
Correlation between off-axis illumination and apodized phase-contrast: Two complementary microscopic phase-imaging modes.
Journal of Biomedical Optics 13(5):054067
Pelc R., Ishii N. & Ashley C.C. (2009)
Laser flash photolysis of diazo-2, a caged calcium chelator: The relationship between the extent and rate of smooth muscle relaxation.
Journal of Laser Applications 21(1): 32-38
Griffiths P.J., Isaackson H., Pelc R., Redwood C.S., Funari S.S., Watkins H. & Ashley C.C. (2009)
Synchronous in situ ATPase activity, mechanics, and Ca2+ sensitivity of human and porcine myocardium.
Biophys. J. 97(9): 2503-2512
Burdíková Z., Čapek M., Ostašov P., Machač J., Pelc R., Mitchell E.A.D. & Kubínová L. (2010)
Testate amoebae examined by confocal and two-photon microscopy: Implications for taxonomy and ecotoxicology.
Microsc. Microanal. 16(6): 735-746