Pelc Radek

Pelc Radek Country: Czech Republic
E-mail: radek.pelc@seh.oxon.org

Participation in Working Groups

  • WG1 - Biophysics of cell and tissue structure
  • WG2 - Structural analysis of biomolecules involved in mechanobiology
  • WG3 - New methodologies to study mechanobiology of cells and tissues
  • WG4 - Mechanobiological principles of rare and common diseases

Research Interests

My resesarch focuses on developing microscopic optical contrasting (label-free) modalities to efficiently visualize unstained living cells. These include (1) modulation-relief ("schlieren") microscopy as a competitor of much more costly differential interference contrast (DIC Nomarski) microscopy, and (2) phase-contrast microscopy devoid of 'halo' artifacts otherwise present in commercially available microscopes. Recently I started to engage in (3) Raman microscopy where cell staining is largely contraindicated, and hope to couple it with one of the above-mentioned optical-contrasting modalities [1,2] in order to add a much-needed morphological context to the chemical images Raman microscopy provides.

Technologies offered to other EuroCellNet participants

(1) Modulation-relief ("schlieren") microscopy

(2) Label-free phase-contrast microscopy (under development)

(3) Raman microscopy

Technologies sought from other EuroCellNet participants

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Publications

Pelc R., Hostounský Z., Otaki T. & Katoh K. (2018 in press)

Conventional, apodized and relief phase-contrast microscopy.

In: Neurohistology & Imaging: Basic Techniques (Pelc R., Doucette J.R. & Walz W., Eds.), pp. XXX-XXX. 

(Neuromethods XX: XXX-XXX)

Springer (Humana Press), New York

https://www.researchgate.net/publication/326693064

Pelc R., Hostounský Z. & Otaki T. (2008)

Correlation between off-axis illumination and apodized phase-contrast: Two complementary microscopic phase-imaging modes.
Journal of Biomedical Optics 13(5):054067
http://dx.doi.org/10.1117/1.2966716

Pelc R., Ishii N. & Ashley C.C. (2009)

Laser flash photolysis of diazo-2, a caged calcium chelator: The relationship between the extent and rate of smooth muscle relaxation.
Journal of Laser Applications 21(1): 32-38
http://dx.doi.org/10.2351/1.3071377

Griffiths P.J., Isaackson H., Pelc R., Redwood C.S., Funari S.S., Watkins H. & Ashley C.C. (2009)

Synchronous in situ ATPase activity, mechanics, and Ca2+ sensitivity of human and porcine myocardium.
Biophys. J. 97(9): 2503-2512
http://dx.doi.org/10.1016/j.bpj.2009.07.058

https://www.researchgate.net/publication/38061717

Burdíková Z., Čapek M., Ostašov P., Machač J., Pelc R., Mitchell E.A.D. & Kubínová L. (2010)

Testate amoebae examined by confocal and two-photon microscopy: Implications for taxonomy and ecotoxicology.
Microsc. Microanal. 16(6): 735-746
http://dx.doi.org/10.1017/S1431927610094031

https://www.researchgate.net/publication/49623619