My work has been focused in the extracellular matrix for more that 20 years already, since I initiated my postdoctoral training in the laboratory of Dr Iruela-Arispe (currently at UCLA, USA). At that point I became involved in the identification of extracellular proteases and their implication in angiogenesis processes. Since then to date (currently I enjoy a PI position at research center GENYO, in Granada, Spain), I have been working with different molecules and working models, all of them intimately related with the extracellular microenvironment (thrombospondins, nidogens, syndecans, MMPs, ADAMTS, …), so I feel very close to the aims of this Cost Action.
Currently, my main lines of research are:
- Characterization of the mechanism of action of ADAMTS metaloproteases by its functional interaction with substrates.
- Modulation of tumor plasticity, angiogenesis and immune response within the tumour microenvironment, by the activity of extracellular proteases.
- Transcriptomic analysis in tumor models according to the activity of ADAMTS proteases and their substrates.
- 2D and 3D co-culture models of tumour and endothelial cells.
- Confocal microscopy to evaluate ECM molecules
- Tumour models to study modifications of extracellular matrix constituents.
- Evaluation of specific markers (vasculature, immune, stemness) in human and mouse tumour samples.
- Proteomic approaches to evaluate the status of ECM molecules
- Knowledge of biophysics parameters to correlate them with activity of specific molecules
- Knowledge of mechanobiology concepts
- Knowledge of further ECM components
- Expertise in 2D/3D culture models and organoids
1: Santos-Oliveira P, Correia A, Rodrigues T, Ribeiro-Rodrigues TM, Matafome P,
Rodríguez-Manzaneque JC, Seiça R, Girão H, Travasso RD. The Force at the
Tip--Modelling Tension and Proliferation in Sprouting Angiogenesis. PLoS Comput
Biol. 2015 Aug 6;11(8):e1004436. doi: 10.1371/journal.pcbi.1004436. eCollection
2015 Aug. Erratum in: PLoS Comput Biol. 2015 Nov;11(11):e1004616. PubMed PMID:
26248210; PubMed Central PMCID: PMC4527825.
2: Rodríguez-Manzaneque JC, Fernández-Rodríguez R, Rodríguez-Baena FJ,
Iruela-Arispe ML. ADAMTS proteases in vascular biology. Matrix Biol. 2015
May-Jul;44-46:38-45. doi: 10.1016/j.matbio.2015.02.004. Epub 2015 Feb 17. Review.
PubMed PMID: 25698314.
3: Travasso RD, Corvera Poiré E, Castro M, Rodríguez-Manzaneque JC,
Hernández-Machado A. Tumor angiogenesis and vascular patterning: a mathematical
model. PLoS One. 2011;6(5):e19989. doi: 10.1371/journal.pone.0019989. Epub 2011
May 27. Erratum in: PLoS One. 2011;6(6).
Rodrguez-Manzaneque, Juan Carlos [corrected to Rodríguez-Manzaneque, Juan
Carlos]. PLoS One. 2016;11(1):e0146650. PubMed PMID: 21637756; PubMed Central
4: Reynolds LE, Watson AR, Baker M, Jones TA, D'Amico G, Robinson SD, Joffre C,
Garrido-Urbani S, Rodriguez-Manzaneque JC, Martino-Echarri E, Aurrand-Lions M,
Sheer D, Dagna-Bricarelli F, Nizetic D, McCabe CJ, Turnell AS, Kermorgant S,
Imhof BA, Adams R, Fisher EM, Tybulewicz VL, Hart IR, Hodivala-Dilke KM. Tumour
angiogenesis is reduced in the Tc1 mouse model of Down's syndrome. Nature. 2010
Jun 10;465(7299):813-7. doi: 10.1038/nature09106. Erratum in: Nature. 2010 Jul
15;466(7304):398. PubMed PMID: 20535211; PubMed Central PMCID: PMC3479956.
5: Casal C, Torres-Collado AX, Plaza-Calonge Mdel C, Martino-Echarri E, Ramón Y
Cajal S, Rojo F, Griffioen AW, Rodríguez-Manzaneque JC. ADAMTS1 contributes to
the acquisition of an endothelial-like phenotype in plastic tumor cells. Cancer
Res. 2010 Jun 1;70(11):4676-86. doi: 10.1158/0008-5472.CAN-09-4197. Epub 2010 May
18. PubMed PMID: 20484033.