Our group investigates the function of keratin cytoskeletal proteins in epidermal differentiation and disease, employing cultured keratinocytes, genetically engineered mice and the fly as model systems. In particulary, we focus on the question how individual keratins regulate desmosome adhesion, mitochondrial function and cell mechanics in distinct ways. In addition, we exploit our understanding of basic cell biological methods to develop molecular rational therapies for keratin disorders such as epidermolysis bullosa simplex, which are characterized by formation of protein aggregates. In addition, we investigate potential roles of vimentin in human diseases, aiming to understand whether vimentin and keratins perform analogous or unique functions.
keratinocyte 2D and 3D culture, mouse and fly models, light microscopy (confocal, laser capture, video microscopy), image analysis, molecular cell biology and biochemistry methods
high content library screening, mass spectrometry, super resolution microscopy
A Mashukova, AS Oriolo, FA Wald, Y Figueroa, ML Casanova, C Kroeger, TM Magin, MB Omary and PJ Salas. Rescue of active atypical protein kinase C in epithelia by the cytoskeleton and Hsp70 family chaperones. J Cell Science 122, 2491-2503 (2009).
K Seltmann, A Fritsch, J Käs and TM Magin. Keratins significantly contribute to cell stiffness and impact on invasive behavior. PNAS 110, 18507-18512 (2013).
C Kröger, F Loschke, N Schwarz, R Windoffer, RE Leube and TM Magin. Keratins control intercellular adhesion involving PKCα-mediated desmoplakin phosphorylation. J Cell Biol 201, 681-692 (2013).
V Kumar, M Behr, D Kiritsi, A Scheffschick, A Grahnert, M Homberg, A Schwieger-Briel, L Bruckner-Tuderman and TM Magin. Keratin-dependent TSLP expression suggests a link between skin blistering and atopic disease. J Allergy Clin Immunol. 2016 Nov;138(5):1461-1464.e6. doi: 10.1016/j.jaci.2016.04.046. Epub 2016 Jun 15 (2016).
F Büchau, C Munz, C Has, R Lehmann and TM Magin. KLHL16 degrades epidermal keratins. J Invest Dermatol; Feb 23. pii: S0022-202X(18)30140-4. doi: 10.1016/j.jid.2018.02.017 (2018).