I'm interested in nuclear proteins, especially lamins and emerin and they role in health and disease. My research projects focus on molecular background of laminopathies, lamins regulation and signalling as well as on therapeutic approach in Emery Dreifuss Muscular Dystrophy.
I have expertise in cell culture techniques such as primary and immortalized cell lines, transgenes expression, transfection techniques, viral vectors and live cell analysis. I work mostly with muscle cells. I also have experience with microscopy techniques, immunostaining, biochemistry analyses, gene expression measurements, flow cytometry, cloning and othe molecular biology techniques.
I'm interested in different aspects of cell nucleus and I'm especially interested in techniques focused on nuclear lamina, such as investigating interactions network and gene expression regulation.
Pałka M, Tomczak A, Grabowska K, Machowska M, Piekarowicz K, Rzepecka D, Rzepecki R (2018): „Laminopathies - what can humans learn from fruit fly" CMBL
Piekarowicz K, Machowska M, Dratkiewicz E, Lorek D, Madej-Pilarczyk A, Rzepecki R (2016): „The effects of the lamin A and its mutants on nuclear structure, cell proliferation, protein stability and mobility in embryonic cells.”, Chromosoma
Machowska M*, Piekarowicz K*, Rzepecki R (2015): „Regulation of lamin properties and functions – does phosphorylation do it all?” Open Biology (*equally contributed)
Zaremba-Czogalla M, Piekarowicz K, Wachowicz K, Kozioł K, Dubińska-Magiera M, Rzepecki R (2012): “The different function of single phosphorylation sites of Drosophila melanogaster lamin Dm and lamin C” PLoS One 7(2):e32649