Sztacho Martin

Country: Czech Republic
Laboratory webpage
E-mail: martin.sztacho@img.cas.cz

Participation in Working Groups

  • WG1 - Biophysics of cell and tissue structure

Research Interests

Phosphoinositides (PIs) are important regulators of cellular signaling. Differently phosphorylated PIs specifically recruit proteins through recognition modules like PH domains. The protein recruitment increases the rate of multimeric protein complexes formation due to reduction of 3D free solution environment onto 2D world on the membrane surface. Such phenomenon is obvious in the regulation of osteoclast polarity during a bone turnover, the mechanism I have studies during my PhD studies in Germany. Another example of PIs membrane code regulating signaling pathway is during selective autophagy, which I have been studying during my postdoctoral stay in Austria. Finally, the regulatory mechanisms where different PIs provide a further step of compartmentalisation are situated in eukaryotic nucleus. In the laboratory of Prof. Hozak, we are trying to depict the function of different PIs within the cell nucleus.

Technologies offered to other EuroCellNet participants

Cell culture – osteoclasts, invasive cancerous lines, transfection and transduction of terminally diff. cell types, electroporation, siRNA, preparation of mineralized and other coated surfaces, migration assays, anoikis assays, SILAC labelling, adenovirus and lentivirus production and handling (S2)

Imaging – immunofluorescence staining, TIRF, confocal, live-cell imaging

Image processing – Cell Profiler, Fiji, Photoshop, PyMOL

Lipid analysis – lipid extraction from cells, PIP3 staining and measurement, TLC, GUV preparation

Molecular biology – DNA cloning, RNA handling, PCR, qPCR, RT PCR, restriction, recombineering, CRISPR

Mouse work – mouse handling, colony management, alizarin/alcian staining, immunohistochemistry

Protein biochemistry – WB, affinity purification, GST pull-down, IP, co-IP, in vitro phosphoryaltion, gel filtration, sample preparation for Mass spectrometry

Technologies sought from other EuroCellNet participants

I am willing to broaden my experties in biochemical methods with emphasis to biophysical approaches evaluating the properties of cellular strucutres and processes. Furthermore I would appretiate if EuroCellNet will lead to the connection of other laboratories and institutions, where we could present and discuss our results and other outcomes. Finally I hope that partnership among EuroCellNet participants will give me valuable experience with organising the meetings and discussions together with managing and mentoring experinece.

Publications

Jain A, Vale RD. RNA phase transitions in repeat expansion disorders. Nature. 2017 Jun 8;546(7657):243-247. doi: 10.1038/nature22386. Epub 2017 May 31. PubMed PMID: 28562589; PubMed Central PMCID: PMC5555642.

Cho WK, Jayanth N, Mullen S, Tan TH, Jung YJ, Cissé II. Super-resolution imaging of fluorescently labeled, endogenous RNA Polymerase II in living cells with CRISPR/Cas9-mediated gene editing. Sci Rep. 2016 Oct 26;6:35949. doi: 10.1038/srep35949. PubMed PMID: 27782203; PubMed Central PMCID: PMC5080603.

Ge L, Melville D, Zhang M, Schekman R. The ER-Golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis. Elife. 2013 Aug 6;2:e00947. doi: 10.7554/eLife.00947. PubMed PMID: 23930225; PubMed Central PMCID: PMC3736544.

Portal MM, Pavet V, Erb C, Gronemeyer H. Human cells contain natural double-stranded RNAs with potential regulatory functions. Nat Struct Mol Biol. 2015 Jan;22(1):89-97. doi: 10.1038/nsmb.2934. Epub 2014 Dec 15. PubMed PMID: 25504323.

Banani SF, Lee HO, Hyman AA, Rosen MK. Biomolecular condensates: organizers of cellular biochemistry. Nat Rev Mol Cell Biol. 2017 May;18(5):285-298. doi: 10.1038/nrm.2017.7. Epub 2017 Feb 22. Review. PubMed PMID: 28225081.