Hozák Pavel

Hozák Pavel Country: Czech Republic
Laboratory webpage
E-mail: pavelhozak@gmail.com

Participation in Working Groups

  • WG1 - Biophysics of cell and tissue structure
  • WG2 - Structural analysis of biomolecules involved in mechanobiology
  • WG3 - New methodologies to study mechanobiology of cells and tissues
  • WG4 - Mechanobiological principles of rare and common diseases

Research Interests

Nuclear interior is functionally highly compartmentalized, and the recent evidence points strongly to structure-related regulation of nuclear functions – however, the mechanisms forming the 3D-structure of the nucleus are still mostly obscure. We therefore employ a multi-disciplinary approach in order to study nuclear functions in relation to the higher-order nuclear structures, e.g. nuclear bodies, the nucleolus, and the nucleoskeleton. Our research concentrates on: (1) the relationship between nuclear compartmentalization and regulation of gene expression, (2) structure, dynamics, and function of the nucleoskeleton, which contributes to the nuclear compartmentalization, (3) functions of nuclear myosins and actins in transcription and gene expression, (4) functions of nuclear lipids, (5) development of new microscopy methods.

Technologies offered to other EuroCellNet participants

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Technologies sought from other EuroCellNet participants

Structural analysis and modelling of molecular interactions for nuclear proteins

Publications

1: Venit T, Kalendová A, Petr M, Dzijak R, Pastorek L, Rohožková J, Malohlava J,
Hozák P. Nuclear myosin I regulates cell membrane tension. Sci Rep. 2016 Aug
2;6:30864. doi: 10.1038/srep30864. PubMed PMID: 27480647; PubMed Central PMCID:
PMC4969604.

2: Pastorek L, Sobol M, Hozák P. Colocalization coefficients evaluating the
distribution of molecular targets in microscopy methods based on pointed
patterns. Histochem Cell Biol. 2016 Oct;146(4):391-406. doi:
10.1007/s00418-016-1467-y. PubMed PMID: 27460592; PubMed Central PMCID:
PMC5037163.

3: Marášek P, Dzijak R, Studenyak I, Fišerová J, Uličná L, Novák P, Hozák P.
Paxillin-dependent regulation of IGF2 and H19 gene cluster expression. J Cell
Sci. 2015 Aug 15;128(16):3106-16. doi: 10.1242/jcs.170985. PubMed PMID: 26116569;
PubMed Central PMCID: PMC4541046.

4: Kalendová A, Kalasová I, Yamazaki S, Uličná L, Harata M, Hozák P. Nuclear
actin filaments recruit cofilin and actin-related protein 3, and their formation
is connected with a mitotic block. Histochem Cell Biol. 2014 Aug;142(2):139-52.
doi: 10.1007/s00418-014-1243-9. PubMed PMID: 25002125; PubMed Central PMCID:
PMC4110419.

5: Sobol M, Yildirim S, Philimonenko VV, Marášek P, Castaño E, Hozák P. UBF
complexes with phosphatidylinositol 4,5-bisphosphate in nucleolar organizer
regions regardless of ongoing RNA polymerase I activity. Nucleus. 2013
Nov-Dec;4(6):478-86. doi: 10.4161/nucl.27154. PubMed PMID: 24513678; PubMed
Central PMCID: PMC3925692.

6: Philimonenko VV, Philimonenko AA, Šloufová I, Hrubý M, Novotný F, Halbhuber Z,
Krivjanská M, Nebesářová J, Šlouf M, Hozák P. Simultaneous detection of multiple
targets for ultrastructural immunocytochemistry. Histochem Cell Biol. 2014
Mar;141(3):229-39. doi: 10.1007/s00418-013-1178-6. Erratum in: Histochem Cell
Biol. 2014 Mar;141(3):241. PubMed PMID: 24449180; PubMed Central PMCID:
PMC3935117.

7: Venit T, Dzijak R, Kalendová A, Kahle M, Rohožková J, Schmidt V, Rülicke T,
Rathkolb B, Hans W, Bohla A, Eickelberg O, Stoeger T, Wolf E, Yildirim AÖ,
Gailus-Durner V, Fuchs H, de Angelis MH, Hozák P. Mouse nuclear myosin I
knock-out shows interchangeability and redundancy of myosin isoforms in the cell
nucleus. PLoS One. 2013 Apr 11;8(4):e61406. doi: 10.1371/journal.pone.0061406.
PubMed PMID: 23593477; PubMed Central PMCID: PMC3623870.

8: Dingová H, Fukalová J, Maninová M, Philimonenko VV, Hozák P. Ultrastructural
localization of actin and actin-binding proteins in the nucleus. Histochem Cell
Biol. 2009 Mar;131(3):425-34. doi: 10.1007/s00418-008-0539-z. PubMed PMID:
19039601.

9: Janderová-Rossmeislová L, Nováková Z, Vlasáková J, Philimonenko V, Hozák P,
Hodný Z. PML protein association with specific nucleolar structures differs in
normal, tumor and senescent human cells. J Struct Biol. 2007 Jul;159(1):56-70.
PubMed PMID: 17428679.

10: Castano E, Philimonenko VV, Kahle M, Fukalová J, Kalendová A, Yildirim S,
Dzijak R, Dingová-Krásna H, Hozák P. Actin complexes in the cell nucleus: new
stones in an old field. Histochem Cell Biol. 2010 Jun;133(6):607-26. doi:
10.1007/s00418-010-0701-2. Review. PubMed PMID: 20443021.

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