Cellular and molecular basis of diabetes, regulatory proteins and genes in development of diabetes; determinations of the proteins and/or mRNAs involved in pancreatic beta cell regeneration and apoptosis, molecular pathology of the complications caused by diabetes in a variety of organs. The investigation of the relation between the clinical properties and genotypes of some susceptibility genes in type II diabetic patients. The running projects include the investigations on beta cell regeneration, apoptosis and stem cells; the regenerative effects of DPP4 inhibitors on islets cells and liver cells in experimental diabetes.
The molecular basis of cancer including the oncogenes, programmed cell death (apoptosis and autophagic cell death) and metastase related genes in patients with breast and ovarian cancers, and in cancer cell lines.
The studies based on nervous system include the taupathy models (Alzheimer’s disease) in primary neuron culture and the in vivo experimental models of medulla spinalis trauma. The running projects is on the Pituitary adenomas and acromegaly disease; to identify relationship between acromegaly patient’s response to treatment and genetic variations in the miRNA genes controlling the gene expression of HMGA2, AIP and SSTR2.
Immunohistochemistry, Immunogold, in situ hybridization, TUNEL, Enzyme histochemistry, ELISA, Western blotting, Light and Electron microscopy (TEM), Cell cultures, in vivo experimental models such as diabetes, neurodegeneration and hyperlipidemia. Molecular biology and genetic analysis ( e.g.PCR, qRT-PCR, RLFP, ect.)
Confocal microscopy, Correlative light and electron microscopy, Light microscopy super resolution, in vivo imaging, immunuprescipitation, advanced gene editing techniques, tissue array, microarrey-mRNA, bioinformatics.
Metin-Armağan D, Gezen-Ak D, Dursun E, Atasoy İ, Karabay A, Yılmazer S, Öztürk M. The effect of tau hyperphosphorylation on Pin1 expression in okadaic acid– induced primary cortical neurons. Submitted to “ Acta Histochemica”
Özsöylemez ÖD, Öztürk M, Sütlüpinar N, Kayacan S, Tunçdemir M, Özcan G. The Effects of Colchicum baytopiorum on Regulatory Genes of Apoptotic and Autophagic Cell Death in HeLa Cells.Curr Pharm Biotechnol. 2016 Nov 9. [Epub ahead of print]
Tunçdemir M, Öztürk M. Regulation of the Ku70 and apoptosis-related proteins in experimental diabetic nephropathy. Metabolism. 65(10):1466-77. 2016
Dagistanli FK, Ozkaya HM, Kucukyoruk B, Biceroglu H, Metin D, Gazioglu N, Oz B, Kadioglu P, Ozturk M. Preoperative Somatostatin Analogue Treatment Might Trigger Apoptosis and Autophagy in Tumor Tissues of Patients with Acromegaly: A Pilot Study. Exp Clin Endocrinol Diabetes. 2016 Jun 20. [Epub ahead of print]
Kaya-Dagistanli F, Ozturk M. The role of clusterin on pancreatic beta cell regeneration after exendin-4 treatment in neonatal streptozotocin administrated rats. Acta Histochem. Jul;115(6):577-86. 2013
Kaya-Dagistanli F, Tanriverdi G, Altinok A, Ozyazgan S, Ozturk M. The effects of alpha lipoic acid on liver cells damages and apoptosis induced by polyunsaturated fatty acids. Food Chem Toxicol Mar;53: 84-93. 2013
Duman BS, Cagatay P, Hatemi H, Ozturk M. Association of resistin gene 3'-untranslated region EX4-44G-A polymorphism with obesity- and insulin-related phenotypes in turkish type 2 diabetes patients. Rev Diabet Stud. 4(1):49-55, 2007