Weihs Daphne

Country: Israel
Laboratory webpage
E-mail: daphnew@technion.ac.il

Participation in Working Groups

  • WG1 - Biophysics of cell and tissue structure
  • WG3 - New methodologies to study mechanobiology of cells and tissues
  • WG4 - Mechanobiological principles of rare and common diseases

Research Interests

Our focus is on the cell mechanobiology with regard to disease and health, with special focus on cancer and wound healing. We study the effects of substrate stiffness on cell motility and spreading, in relation to metastasis formation and wound closure. We use mechanics to identify mechanisms of force application, reveal inherent mechanical differences between cancer and benign, and evaluate treatments for prevention of cancer metastasis.
We perform experiments, develop analysis tools including advanced image analysis, and perform finite element modeling of our systems.

Technologies offered to other EuroCellNet participants

Traction force microscopy in 2D and 3D to evaluate cell-substrate interactions
Particle tracking microrheology, Bio-microrheology
Real-time, high-resolution fluorescence microscopy with combined time-lapse.
Various cell biology techniques (immunofluorescence, viability and proliferation, etc.).
Image processing for high-resolution imaging in 2D and 3D
Finite element analysis and modeling

Technologies sought from other EuroCellNet participants

Advanced mechanobiology approaches to evaluate biological interactions during mechanobiology

Finite element analysis and modeling

Publications

Revital Kristal-Muscal, Liron Dvir, and Daphne Weihs, “Metastatic cancer cells tenaciously indent impenetrable, soft substrates”, New Journal of Physics 15, 035022 (2013). DOI: 10.1088/1367-2630/15/3/035022.

Diana Goldstein, Tal Elhanan, Maria Aronovitch, and Daphne Weihs, “Origin of active transport in breast-cancer cells”, Soft Matter 9(29), 7167-7173 (2013). DOI: 10.1039/C3SM50172H.

Liron Dvir, Ronen Nissim, Martha B. Alvarez-Elizondo, and Daphne Weihs, “Quantitative measures to reveal coordinated cytoskeleton-nucleus reorganization during in vitro invasion of cancer cells”, New Journal of Physics 17, 043010 (2015).

Shada Abuhattum* and Daphne Weihs, “Asymmetry in produced traction forces in migrating preadipocytes is bounded to 33%”, Medical Engineering and Physics 38(9), 834-838 (2016).

Samer Toume, Amit Gefen, and Daphne Weihs, “Low-level stretching accelerates cell migration into a gap”, In Press International Wound Journal (Epub 17 October 2016). PMID: 27748039, DOI: 10.1111/iwj.12679.