We are interested to improve the understanding about role of the major macroglia in the retina, the Müller cells, in pathomechanisms of the retina. We perform RNA sequencing and proteomic profiling on Müller cells sorted from retinae of different species (mouse, pig, human) and compare the expression profiles from different pathologies (ischmic damage, diabetes, light damage) and to other cellular entities of the retina (microglia, endothlial cells, neurons). Valdiation of candidate genes is performed by standard molecular biology and immunolabeling as well ass physiological tools such as measurements of Müller cell volume regulation, transmitter release and calcium imaging. Using AAV vectors we try to modulate Müller cell gene expression to maintain their neuron-supportive functions in the diseased retina.
Magnetic activated cell sorting of retinal cell types
Imaging of glutamate release, calcium imaging, measurement of cell volume regulation
Bioinformatic analysis techniques, novel imaging techniques, animal models
9-11 September 2019, Trnava, Slovakia