Malakauskas Kestutis

Malakauskas Kestutis Country: Lithuania
E-mail: kestutis.malakauskas@lsmuni.lt

Participation in Working Groups

  • WG1 - Biophysics of cell and tissue structure
  • WG4 - Mechanobiological principles of rare and common diseases

Research Interests

Our research interests - evaluation of changes in cell-cell and cell-extracellular matrix (ECM) interactions during chronic lung diseases (asthma, COPD, idiopathic pulmonary fibrosis). The aims - to investigate how inflammatory cells (mainly, eosinophils, monocytes) dysregulate release of ECM proteins by airway smooth muscle cells and pulmonary fibroblasts and how released ECM proteins change behavior of structural cells. Additionally, we are evaluating the possible signaling pathways (Wnt, Smad) being involved and triggered by eosinophils and ECM proteins thus contributing to airway remodeling.

Technologies offered to other EuroCellNet participants

Our laboratory is closely related with patients with respiratory diseases as it is located in the Department of Pulmonology. We can offer to collaborators special human materials like blood serum samples, whole blood lysates, whole blood mRNA samples, induced sputum samples, protein lysates from cell culture. Moreover, we can offer to accomplish experiments in our laboratory using combined cells culturing methods (inflammatory cells as eosinophils, monocytes with structural cells as airway smooth muscle cells and pulmonary fibroblasts).

Technologies sought from other EuroCellNet participants

We are interested in analysis of ECM proteins and MMP’s clustered genes together with microarray analysis of these genes expression. We expect to find partners who can offer analysis of large scale of clustered genes expression which could help to investigate ways how inflammatory cells communicate with structural cells. We are looking for partners who are interested and have an equipment for detection of compartment of cell receptors responsible for connection with ECM proteins. Therefore, we would like collaborate with researchers who are working on investigation of special materials and drugs which may control cell atachment to ECM proteins fillaments.  

STSM’s and trainings would be a priority for our research group.

Publications

  1. Januskevicius A, Gosens R, Sakalauskas R, Vaitkiene S, Janulaityte I, Halayko AJ, Hoppenot D, Malakauskas K. Suppression of Eosinophil Integrins Prevents Remodeling of Airway Smooth Muscle in Asthma. Frontiers in Physiology, 2017; 7: 680.
  2. Januskevicius A, Vaitkiene S, Gosens R, Janulaityte I, Hoppenot D, Sakalauskas R, Malakauskas K. Eosinophils enhance WNT-5a and TGF-β1 genes expression in airway smooth muscle cells and promote their proliferation by increased extracellular matrix proteins production in asthma. BMC Pulm Med. 2016; 16: 94.
  3. Hoppenot D, Malakauskas K, Lavinskiene S, Sakalauskas R. p-STAT6, PU.1, and NF-κB are involved in allergen-induced late-phase airway inflammation in asthma patients. BMC Pulm Med. 2015; 15: 122.
  4. Lavinskiene S, Malakauskas K, Jeroch J, Hoppenot D, Sakalauskas R. Functional activity of peripheral blood eosinophils in allergen-induced late-phase airway inflammation in asthma patients. J Inflamm. 2015; 12: 25.
  5. Lavinskiene S, Bajoriuniene I, Malakauskas K, Jeroch J, Sakalauskas R. Sputum neutrophil count after bronchial allergen challenge is related to peripheral blood neutrophil chemotaxis in asthma patients. Inflamm Res. 2014; 63: 951-9. 

News

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