As for cellular networks, I am particularly interested and would like to obtain partners in the topology of the chromatin network. The idea is not of local properties but of the whole genome topological (positional) regulation by constitutive heterochromatin and nucleoli, where some biophysical or physico-chemical principles of dissipative systems could be applied.
Among other interests, I am cancer cell biologist, working for last 15 years on the role of reversible polyploidy in resistance of cancer cells to chemotherapy. We found that this inducible and reversible polyploidy also induces the embryonal stemness and proposed a cancer cell ļife-cycle'hypothesis. Our experimental data on the role of polyploidy in cancer were confirmed in many laboratories and recognised. My main expertise are the cytological and cytometric methods.
I do not possess any particularly advanced methods
Composition of FISH and immunofluorescence and life-imaging
Salmina K, Jankevics E, Huna A, Perminov D, Radovica I, Klymenko T, Ivanov A, Jascenko E, Scherthan H, Cragg MS, Erenpreisa J. Up-regulation of the embryonic self-renewal network through reversible polyploidy in irradiated p53-mutant tumour cells.
Exp. Cell Res 2010, 316:2099-112
Schwarz-Finsterle J, Scherthan H, Huna A, González P, Mueller P, Schmitt E, Erenpreisa Je, Hausmann M. Increase and spatial shifts of chromosome territories in nuclei of radiation-induced polyploidising tumour cells. Mut Res 2013, 756: 56-65.
B. I. Gerashchenko, K. Salmina, J. Eglitis, A. Huna, V. Grjunberga, J. Erenpreisa Disentangling the aneuploidy and senescence paradoxes: a study of triploid breast cancers non-responsive to neoadjuvant therapy. Histochemistry and Cell Biology 2016, 45: 497-508
Erenpreisa J, Krigerts J, Salmina K. Radial, nucleolus-dependent gradient of the chromatin distribution in active and aging cells. EMBO Conference: From Functional Genomics to Systems Biology (Heidelberg, 12-15 November, 2016)
Tchuchiya M, Giuliani A, Hashimoto M, Erenpreisa J, Yoshikawa K. Self-Organizing Global Gene Expression Regulated Through Criticality: Mechanism of the Cell-Fate Change. PlosOne (minor revision ) BioRxiv https://www.researchgate.net/deref/http%3A%2F%2Fdx.doi.org%2F10.1101%2F066498 .