The Medalia group at the Department of Biochemistry of the University of Zurich focuses on cellular remodeling of macromolecules and structure-function relations of large protein machines during cellular processes, using three-dimensional (3D) microscopy approaches (Electron microscopy, AFM and light microscopy). In particular, we are interested in the structural organization of the nuclear envelope, i.e. the nuclear pore complex which we already reconstructed in situ which we recently calculated the structure at 20 Å resolution, as well as nuclear lamins [57-60]. Additionally, we are interested in cytoskeleton remodeling during physiological processes, e.g., cell adhesion . This work is primarily conducted by means of cryo-electron tomography of eukaryotic cells, a field we have been pioneering during the last decade . Recently, we established a set of AFM experiments in order to correlate structure and physical properties of the biological samples we study. Thus, by using cryo-electron microscopy and tomography in conjunction with life-cell fluorescence microscopy and AFM, we are able to get insight into the physical properties of cellular processes and macromolecular complexes.
Fast AFM imaging
Sorrentino, S., Studt, JD., Bokstad Horev, M., Medalia, O. & Sapra, KT. Toward correlating structure and mechanics of platelets. Cell Adh Migr [Epub ahead of print] (2016). PMID: 27104281.
Irobalieva, RN., Martins, B. & Medalia, O. Cellular structural biology as revealed by cryo-electron tomography. J. Cell. Sci. 129, 469-76 (2016).
Tamir, A., Sorrentino, S., Motahedeh, S., Shai, E., Dubrovsky, A., Dahan, I., Eibauer, M., Studt, JD., Tanuj Sapra, K., Varon, D. & Medalia, O. The macromolecular architecture of platelet-derived microparticles. J. Struct. Biol. 193, 181-7 (2016)
Eibauer, M., Pellanda, M., Turgay, Y., Dubrovsky, A., Wild, A. & Medalia, O. Structure and gating of the nuclear pore complex. Nat Commun 6, 7532 (2015).
Harapin, J., Börmel, M., Sapra, KT., Brunner, D., Kaech, A. & Medalia, O. Structural analysis of multicellular organisms with cryo-electron tomography. Nat. Methods 12, 634-6 (2015).