Ramiro de Maeztu, 9
Our group has been working for several years on the modification of proteins by electrophilic lipids (protein lipoxidation), which is increased in pathological situations associated with oxidative stress and can drastically alter protein function. We have identified targets of lipoxidation and characterized the structural and functional consequences of the modification. The major constituents of the cytoskeleton, including actin, tubulin and vimentin, are targets for these modifications. In some cases, characterization of their modification has led to discern the important role of the target residues in the regulation of protein function under basal and stress conditions. We are interested in exploring new mechanisms of regulation of cytoskeletal proteins, mainly in response to electrophilic species, with a focus on intermediate filaments. We are very much interested in broadening our view of this field and incorporating new methodologies.
LEICA TCS SP5
LEICA TCS SP2
Leica AF6000 LX
Access to Leica TCS SP8 system with a 3X STED module
JEOL transmission electron microscope JEM-1230
Our group employs molecular and cellular biology techniques. We mainly employ cultured cells transfected with expression constructs of the proteins of interest that are monitored by fluorescence microcopy or used for various cellular assays. We have experience in basic proteomic techniques. We also carry out in vitro assays with recombinant proteins. More detailed information can be gathered from our publications (please see the webpage of the group above).
Posttranslational modification of proteins by reactive species, including endogenous mediators, environmental agents and drugs. Role in physiology and pathophysiology.
Intermediate filament proteins as targets of lipoxidation.
We do not run any facility on our own. Facilities at our institue can be accessed by external users as described in www.cib.csic.es.
25–30 May 2020, Spetses Island, Greece