Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas

Department of Structural and Chemical Biology

Contacts

Head

Dolores Pérez-Sala
Phone:
Email: dperezsala@cib.csic.es

Involved in

  • WG1 - Biophysics of cell and tissue structure

General description research focus

Our group has been working for several years on the modification of proteins by electrophilic lipids (protein lipoxidation), which is increased in pathological situations associated with oxidative stress and can drastically alter protein function. We have identified targets of lipoxidation and characterized the structural and functional consequences of the modification. The major constituents of the cytoskeleton, including actin, tubulin and vimentin, are targets for these modifications. In some cases, characterization of their modification has led to discern the important role of the target residues in the regulation of protein function under basal and stress conditions. We are interested in exploring new mechanisms of regulation of cytoskeletal proteins, mainly in response to electrophilic species, with a focus on intermediate filaments. We are very much interested in broadening our view of this field and incorporating new methodologies.

Imaging technologies available

  • Spinning Disc Confocal Microscopy (SDCM)
  • Stimulated Emission Depletion Microscopy (STED)
  • Fluorescence Recovery After Photobleaching (FRAP)
  • Electron Microscopy
  • Live cell imaging

Equipment available

LEICA TCS SP5

LEICA TCS SP2

Leica AF6000 LX

Access to Leica TCS SP8 system with a 3X STED module

JEOL transmission electron microscope JEM-1230

Expertise support provided to users

Our group employs molecular and cellular biology techniques. We mainly employ cultured cells transfected with expression constructs of the proteins of interest that are monitored by fluorescence microcopy or used for various cellular assays. We have experience in basic proteomic techniques. We also carry out in vitro assays with recombinant proteins. More detailed information can be gathered from our publications (please see the webpage of the group above).

Research topics directly investigated in research group

Posttranslational modification of proteins by reactive species, including endogenous mediators, environmental agents and drugs. Role in physiology and pathophysiology.

Intermediate filament proteins as targets of lipoxidation.

Access rules research groups

We do not run any facility on our own. Facilities at our institue can be accessed by external users as described in www.cib.csic.es.