Faculty of Biology University of Belgrade

The Centre for Laser Microscopy

Address

Studentski trg 16
11000 Belgrade
Serbia

Phone:
www.bio.bg.ac.rs
clm.bio.bg.ac.rs

Contacts

Head

Pavle Andjus
Phone: (00381 11) 30 32 356
Email: clm@bio.bg.ac.rs

Involved in

  • WG1 - Biophysics of cell and tissue structure
  • WG4 - Mechanobiological principles of rare and common diseases

General description research focus

The main research area: Neurophysiology and neurobiophysics of neurological disorders

Research focus:

  • cellular and biophysical basis of neurodegenerative processes and new experimental therapies
  • biophysical profiling of molecular and cellular markers in well-characterized in vitro (neuronal and astrocytes cell culture) and in vivo models (e.g. transgenic ALS rat, rat model of ischemia, tenascin C -/- mice)
  • studies of experimental models of neurodegenerative diseases and conditions are performed by using the range of state-of-the-art biophysical techniques based on live cell imaging (confocal and high speed time resolved videomicroscopy), supported by "patch clamp" electrophysiology, and diverse immunocytochemical approaches

Imaging technologies available

  • Laser Scanning Confocal Microscopy (LSM / CLSM)
  • Live cell imaging

Equipment available

Carl Zeiss LSM 510 system with Axioskop 2FS mot microscope (Ar 457/488/514, HeNe 543, HeNe 633 nm), “Visitron” system: Carl Zeiss Axio Observer A1 inverted microscope with "Evolve" EM 512 digital camera and High Speed Polychromatic Illumination System (300-650 nm) and three Chroma filter sets (Fura, FITC & TRITC)

Expertise support provided to users

confocal microscopy on cell cultures and tissue samples, multichannel fluorescence, 3D reconstruction, surface profiling, dynamical measurements of changes in ion concentrations and production of free radicals, sample preparation (immunohistochemistry, immunocytochemistry), cell cultures and live cell imaging

Research topics directly investigated in research group

  • Cellular mechanisms of neurodegenerative diseases and conditions
  • Neurobiophysics of dynamic interactions between neurons and glia
  • Biomarkers in neurodegenerative processes
  • The role of extracellular matrix in structural plasticity

keywords: neurodegenerative diseases, amyotrophic lateral sclerosis (ALS), cerebral ischemia, extracellular matrix, inflammation, oxidative stress, intracellular calcium

Access rules research groups

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