Currently the group is involved in two main directions: (1) Resistance of cancer cells to genotoxic modalities; (2) A large-scale cell nucleus organisation.
Leitz Ergolux L03-10 microscope equipped with Sony DXC 390P colour video camera. The institute is also equipped by flow cytometer, TEM (not new), and the devices for qPCR and next generation sequencing, and is going to buy a new Zeiss confocal microscope.
We have good expertise in the in vitro models of various genotoxic treatments of cancer cells, their monitoring, etc. We have good expertise in DNA quantative cytochemistry, DNA structural tests, immunocytochemistry, and some in cell nucleus image analysis (Image Pro Plus, Image J), apply FISH, and also study transcription by molecular and bioinformatic methods (in collaboration).
1) Currently we are investigating the interplay between cell senescence and self-renewal in genotoxically treated breast and ovarian cancer cells, epithelial-mesenchymal shift and 'amoeboidisation"of induced polyploids and their resqued descendants, the role of autophagy in resistence of cancer cells to genotoxocity. We have published about 25 experimental articles and reviews showing that resistance is realised through adaptive reversible ploidy cycles, which are in fact asexual reproduction life cycles evolved in early eukaryotes.
2) We have restarted the studies interrupted in 90-ths on the large scale chromatin organisation, currently with stress on the chromatin phase transitions and the roles of heterochromatin and nucleolus in the spatial/temporal information transfer. We are interested in the transcription pulsations, nuclear traffic (with the role of cytoskeleton elements), for which have published more recently some preliminary data and ideas (a few published and submitted articles). Here we should desire collaboration, mutual grant appllication, and help in live and 4D imaging.
We do not have meanwhile the rules for external service. Such things should be settled with our administration.
9-11 September 2019, Trnava, Slovakia