Centro Nacional de Investigaciones Cardiovasculares (CNIC)/ Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12)

Lamins in the Immune System Laboratory (LamImSys)


Calle Melchor Fernández Almagro 3// Hospital 12 de Octubre, Avenida de Andalucia S/N
28029/28041 Madrid

www.cnic.es // imas12.es



Jose Maria Gonzalez-Granado
Phone: +34 914531200 (ext 1503)
Email: jmgonzalezgranado@gmail.com

Involved in

  • WG4 - Mechanobiological principles of rare and common diseases

General description research focus

A-type lamins are nuclear envelope proteins that maintain mechanical stability of the nucleus and communicate the nucleus with the cytoskeleton and the plasma membrane. These proteins are linked to the regulation of the cytoskeleton, signal transduction, higher-order chromatin organization, DNA repair and replication, gene transcription, nuclear positioning, and cell proliferation, differentiation and migration. Although there is increasing knowing about A-type lamins functions in a variety of cell types, its role in the immune system is almost unknown. Among other immune cells, T-cells and some innate immune cells play central roles in orchestrating the protection against numerous and extremely diverse microbial pathogens and have acquired increased relevance in the origin and amelioration of chronic inflammatory diseases. My laboratory is interested in understanding the role of A-type lamins in adaptive and innate immune responses against pathogen infections and chronic inflammatory disorders not only from the cellular point of view but also from the molecular effect that A-lamins may have in the diverse cells types of the immune system

Imaging technologies available

  • Laser Scanning Confocal Microscopy (LSM / CLSM)
  • Multiphoton Microscopy Systems
  • Total Internal Reflection Fluorescence Microscopy (TIRF)
  • Fluorescence Lifetime Imaging Microscopy (FLIM)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)

Equipment available

MaiTai DS laser-Zeiss LSM 780 Upright, MaiTai DS laser–Nikon-ECLIPSE-inverted_ISS-ALBA, Leica SP5-Inverted Confocal on an inverted microscope Leica DMI 6000B, IKON A1-R-Inverted Confocal, Zeiss LSM 700- Inverted Confocal, Leica SPE- Upright Confocal, Zeiss Axiover 200M SP LSM5 ApoTome Inverted, Nikon ECLIPSE 90i- Upright, Nikon ECLIPSE Ti -Time Lapse Inverted, Leica TIRF Inverted, Olympus IX71- PALM Microlaser Inverted
Two FACSCanto II (BD) incorporating three laser lines (405nm, 488nm and 633nm) able to detect 10 parameters (2+6+2) simultaneously.
A LSRFortessa (BD) equipped with four laser lines (405nm, 488nm, 561nm and 633nm) able to detect 18 parameters (7+4+4+3) simultaneously.
A SP6800 Sony spectral analyzer equipped with 3 laser lines (405nm, 488nm, and 633nm) able to detect spectral signals between 400 and 800nm.
A digital flow sorter FACSAria SORP (Becton Dickinson) equipped with 6 lasers (355n, 405nm, 449nm, 488nm, 561nm y 640nm) able to analyze 20 parameters simultaneously. The system is housed in a BSL2 biosecurity hood.
Sy3200 flow sorter (SonyBiotechnology) including two independent sorting modules, one equipped with 4 lasers (405nm, 488nm, 561nm and 640nm) and 16 detectors and an additional one including 2 lasers (488nm and 640nm) and 8 detectors. The system is housed in a BSL2 biosecurity hood.
An automated magnetic sorter (Automacs).

Expertise support provided to users

Microscopy Unit: in vivo multicolor confocal imaging with conventional and two-photon excitation and in vivo FLIM, live cell FLIM-FRET, FCS, N&B, GP, and 2-photon imaging, multi-color live cell, immunofluorescence, FRAP and photoactivation. TIRF.

Research topics directly investigated in research group

Dendritic Cell; Lamin A/C; T-cell differentiation; Vaccinia virus; Leishmania major; inflammatory bowel disease; Memory; Adaptive Immunity; Antigen presentation; Immune synapse; T-cell activation.

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