14220 Praha 4
In recent years, my main research interest has been cytoskeleton organization and dynamics, cell adhesion, and cell signaling. Specifically, I focus on plectin-dependent regulation of actin microfilaments, intermediate filament cytoarchitecture, adhesion mediated signaling, and cell motility using molecular cell biology in various cell types derived from transgenic mice models. The current aim of my lab has the following objectives: 1) identification of genes with unique and essential functions in simple epithelia; 2) generation of mouse models with targeted selected genes; and 3) phenotypic characterization of generated mouse models addressing gene functions in healthy and diseased simple epithelia. My research has practical applications to the understanding of liver fibrosis, inflammatory bowel diseases and carcinogenesis.
Confocal microscope Leica TCS SP5 AOBS Tandem
Leica TCS SP8 confocal microscope
Inverted fluorescence microscope Leica DMI6000 with TIRF illumination
Inverted fluorescence microscope Delta Vision Core with laser photo-manipulation
Leica LMD6000 Laser Microdissection Microscope
Leica DM6000 fluorescence microscope
Leica DMI6000 inverted fluorescence microscope
Leica DMI4000B fluorescence microscope
Leica TCS SP8 STED 3X
DeltaVision OMX™ with the Blaze SIM Module, DSSI localization and TIRF illumination
FEI Tecnai G2 20 LaB6 transmission electron microscope
FEI Morgagni 268 Transmission Electron Microscope with Olympus Mega View III Digital Camera
Sample preparation, image analysis
Current research topics:
Analysis of extracellular niche in mouse models of liver fibrosis.
Inflammatory bowel disease in primary sclerosing cholangitis: Prognostic markers and models.
Experimental colitis and colorectal cancer in mouse model of plectinopathy.
The impact of liver-specific plectin deficiency on pathogenesis of liver diseases.
25–30 May 2020, Spetses Island, Greece