Institute of Molecular Genetics CAS

Microscopy Centre

Address

Vídeňská 1083
14200 Prague
Czech Republic

Phone: +420 241 062 219
www.img.cas.cz/en
www.img.cas.cz/core-facilities/microscopy-centre

Contacts

Head

Pavel Hozák
Phone: +420 241 062 219
Email: hozak@img.cas.cz

Involved in

  • WG1 - Biophysics of cell and tissue structure
  • WG2 - Structural analysis of biomolecules involved in mechanobiology
  • WG3 - New methodologies to study mechanobiology of cells and tissues
  • WG4 - Mechanobiological principles of rare and common diseases

General description research focus

The Microscopy Centre offers a wide range of state-of-the-art light and electron microscopy equipment and techniques. The core facility extensively uses the cooperation with industry to continuously develop the new methods and upgrade the available tools.

The centre provides services to both the local researchers and external users. The centre is also active in organizing the training and the courses focused on theoretical and practical aspects of basic and advanced microscopy techniques.

Imaging technologies available

  • Laser Scanning Confocal Microscopy (LSM / CLSM)
  • Deconvolution Widefield Microscopy
  • Total Internal Reflection Fluorescence Microscopy (TIRF)
  • Stimulated Emission Depletion Microscopy (STED)
  • Stochastic Optical Reconstruction Microscopy (STORM)
  • Fluorescence Lifetime Imaging Microscopy (FLIM)
  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Recovery After Photobleaching (FRAP)
  • Electron Microscopy
  • Structured llumination Imaging (SIM) technology
  • Electron tomography
  • Live cell imaging

Equipment available

FEI Tecnai G2 20 LaB6 transmission electron microscope

FEI Morgagni 268 Transmission Electron Microscope with Olympus Mega View III Digital Camera

LEICA EMPACT2 high-pressure freezer preventing the formation of crystal ice

LEICA EM AFS2 processing the samples in automated or manual mode for freeze-substitution and plastic embedding

FEI Vitrobot Mark III

LEICA EM UltraCut6 cutting ultrathin (cryo-) sections 50 - 100 nm and semi-thick sections for tomography (200 – 500 nm)

LKB KnifeMaker Type 7800 to prepare glass knives for ultramicrotomy

E6700 Turbo Molecular Bench Top Vacuum Evaporator

Gold – software tools for ultrastructural studies

BD LSRII flow cytometer, BD FACSCalibur flow cytometer

BD Influx high speed cell sorter

AutoMACS Pro magnetic separator

Confocal microscope Leica TCS SP5 AOBS Tandem

Leica TCS SP8 confocal microscope

Inverted fluorescence microscope Leica DMI6000 with TIRF illumination

Inverted fluorescence microscope Delta Vision Core with laser photo-manipulation

Olympus Cell^R/Scan^R, Leica LMD6000 Laser Microdissection Microscope

Leica DM6000 fluorescence microscope

Leica DMI6000 inverted fluorescence microscope

Leica DMI4000B fluorescence microscope

Leica TCS SP8 STED 3X

DeltaVision OMX™ with the Blaze SIM Module

DSSI localization and TIRF illumination

Tescan Q-PHASE

Multimodal holographic microscope

Expertise support provided to users

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Research topics directly investigated in research group

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Access rules research groups

https://www.czech-bioimaging.cz/